Optimal Aspergillus fumigatus and Asp f 1 serum IgG cut-offs for the diagnosis of allergic bronchopulmonary aspergillosis

The presence of IgG antibodies (Abs) to Aspergillus fumigatus (Af) is a crucial diagnostic criterion for allergic bronchopulmonary aspergillosis (ABPA). Although precipitation traditionally used document Abs, anti-Af serum levels can also be measured by enzyme immunoassay (EIA). However, there are insufficient data on the optimal cut-offs assess performance EIA method. This study aimed determine cut-off binding crude Af extracts or recombinant Asp f 1 (by ImmunoCAP®) and compare their efficacy ABPA diagnosis with Af-precipitating Abs. age distribution (Af-IgG) (Asp 1-IgG) was established using sera from 694 healthy controls (HC). Receiver operating characteristic analysis Af-IgG 1-IgG purpose performed in 306 Af-sensitized asthma patients (including 49 ABPA), were determined. An age-dependent decline observed HC. Thus, determined separately as 60 mg/L aged <55 years, 45 those ?55 years. For 1-IgG, 6.6 set regardless age. such testing allowed sufficiently good performance, Abs had better applicability ABPA. We EIA, which useful clinical settings where precipitating unavailable.